Climbing behavior of 60 day-old flies.

Healthy looking 60 day-old male flies were selected for this assay. Ten flies were placed in a 100 ml glass cylinder. Following a brief and gentle tapping, the distribution of flies was then counted in 3 bins at different heights (above or at 35 mm height, between 7.5 mm and 35 mm, and 7.5 mm or lower). Climbing pattern of flies expressing SOD1 in motoneurons (A), glial cells (B), and co-expressing SOD1 in both motoneurons and glial cells (C) are shown. Flies expressing human SOD1 proteins in glial cells show a significant shift of distribution to the bottom of the cylinder compared to M1B>UAS-dsod1 (the control fly) and in comparison with their expression in motoneurons (A) or dual expression in MNs + glia (C). These observations suggest a significant impairment of climbing activities at 60 days in flies expressing human SOD1s in glia. The statistical significances were calculated using Prizm software, Two-way ANOVA and * p本实验选取外观健康的60日龄雄性果蝇开展检测。将10只果蝇置于100 ml玻璃圆筒中，经轻柔短暂敲击后，按三个不同高度区间对果蝇分布进行计数：高度≥35 mm、7.5 mm~35 mm以及≤7.5 mm。本研究展示了三类果蝇的攀爬行为模式：仅在运动神经元（motoneurons）中表达超氧化物歧化酶1（SOD1）的A组、仅在胶质细胞（glial cells）中表达SOD1的B组，以及同时在运动神经元与胶质细胞中共表达SOD1的C组。与M1B>UAS-dsod1（对照果蝇）相比，同时相较于仅在运动神经元中表达SOD1的A组与运动神经元-胶质细胞共表达SOD1的C组，在胶质细胞中表达人源SOD1蛋白的果蝇，其分布显著向圆筒底部偏移。上述结果表明，60日龄时在胶质细胞中表达人源SOD1的果蝇，其攀爬活动存在显著损伤。统计学显著性检验采用Prism软件进行双因素方差分析（Two-way ANOVA），* p