Spearman rank correlation analysis for pairs of E-cadherin turnover rates.

E-cadherin turnover rates were measured by FRAP as follows: iterative pairs were obtained by measuring E-cadherin turnover rates in a same spot at two time points 45 minutes apart (line 1). Single-cell pairs were obtained by measuring rates simultaneously at two distinct junctions of a same cell (line 2). Single-junction pairs were assessed by dual-color FRAP on E-cadherin from two neighbor cells in a same junction (line 3). Correlations within pairs (between koff#2 and koff#1) were estimated by computing the Spearman coefficient symsymρ. As a comparison to evaluate the strength of the correlation, this result is compared to a randomized situation, obtained by a random permutation within one set of rates (koff#2). Significant correlations (pSpearman rank correlation analysis for pairs of E-cadherin turnover rates.研究人员采用荧光漂白恢复实验（FRAP）测定E-钙粘蛋白（E-cadherin）的周转速率，实验分组方式如下： 1. 迭代配对样本：在同一观测点以45分钟为间隔开展两次测量，以此获取该观测点的E-钙粘蛋白周转速率配对数据； 2. 单细胞配对样本：对同一细胞的两个不同细胞连接同时测定周转速率，以此获取配对数据； 3. 单连接配对样本：针对同一细胞连接内的两个相邻细胞的E-钙粘蛋白，采用双色荧光漂白恢复实验（FRAP）进行检测，以此获取配对数据。 配对样本内的相关性（即koff#2与koff#1之间的相关性）通过计算斯皮尔曼相关系数symsymρ进行评估。为评估相关性的强弱，将上述计算得到的相关系数与随机化场景下的结果进行对比：随机化场景通过对单一组别周转速率（koff#2）进行随机置换得到。随后通过斯皮尔曼等级相关分析，对E-钙粘蛋白周转速率配对样本的相关性显著性进行检验。