Primers used for PCR and in silico evaluation of the specificity.
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aCalculated using NetPrimer (http://www.premierbiosoft.com/netprimer). Tm was estimated using the Nearest neighbor method implemented in the NetPrimer.bPrimers MCGI-554r, 333Fa, ARC915R, Arch958R, and 1017FAR are identical to CREN537, A333F, A934R, A976R, and A1040F, respectively.
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2015-12-02



