Primers used for PCR and in silico evaluation of the specificity.

aCalculated using NetPrimer (http://www.premierbiosoft.com/netprimer). Tm was estimated using the Nearest neighbor method implemented in the NetPrimer.bPrimers MCGI-554r, 333Fa, ARC915R, Arch958R, and 1017FAR are identical to CREN537, A333F, A934R, A976R, and A1040F, respectively.a. 计算过程使用NetPrimer工具（http://www.premierbiosoft.com/netprimer）完成。解链温度（Tm）通过NetPrimer内置的最近邻法进行估算。b. 引物MCGI-554r、333Fa、ARC915R、Arch958R及1017FAR分别与CREN537、A333F、A934R、A976R、A1040F完全一致。