The genetic basis of cytoplasmic male sterility and fertility restoration in wheat

Hybrid wheat varieties give higher yields than conventional lines but are difficult to produce due to a lack of effective control of male fertility in breeding lines. One promising system involves the Rf1 and Rf3 genes that restore fertility of wheat plants carrying Triticum timopheevii-type cytoplasmic male sterility (T-CMS). By genetic mapping and comparative sequence analyses we identified Rf1 and Rf3 candidates that could restore normal pollen production in transgenic wheat plants carrying T-CMS. We show that Rf1 and Rf3 bind to the mitochondrial orf279 transcript and induce cleavage, preventing expression of the CMS trait. The identification of restorer genes in wheat is an important step towards the development of hybrid wheat varieties based on a CMS-Rf system. The characterisation of their mode of action brings new insights into the molecular basis of CMS and fertility restoration in plants.

This dataset includes transcript count and coverage data from 2 RNA-seq experiments l...

杂交小麦品种的产量高于常规品系，但由于无法对育种品系的雄性育性进行有效调控，其培育仍存在较大困难。其中一种颇具应用前景的系统涉及Rf1与Rf3基因，二者可恢复携带提莫菲维小麦型细胞质雄性不育（Triticum timopheevii-type cytoplasmic male sterility, T-CMS）的小麦植株的育性。本研究通过遗传作图与比较序列分析，鉴定得到可恢复携带T-CMS的转基因小麦植株正常花粉生成能力的Rf1与Rf3候选基因。实验证实，Rf1与Rf3可结合线粒体orf279转录本并诱导其切割，从而抑制CMS性状的表达。小麦恢复基因的鉴定，为基于CMS-Rf系统的杂交小麦品种培育迈出了关键一步；而对其作用模式的解析，则为植物细胞质雄性不育与育性恢复的分子机制提供了全新见解。

本数据集包含2项RNA测序实验产生的转录本计数与覆盖度数据……