Quantitative proteomic analysis of ARPE-19 cells in response to LPS

Overwhelming and persistent inflammation of retinal pigment epithelium (RPE) induces destructive changes in retinal environment by invoking immune activation. In this study, we aimed to investigate RPE-specific biological and metabolic response against intense inflammation, and identify molecular characteristics determining pathological progression. Here, we performed quantitative analyses of proteome and phosphoproteome of lipopolysaccharide (LPS)-stimulated human derived RPE cell line ARPE-19 using the latest isobaric TMT labeling approach coupled with high-resolution mass spectrometry.