Analysis of the effect of androgen and androgen receptor on gene expression in CRPC model cell. Analysis of the effect of androgen and androgen receptor on gene expression in CRPC model cell

Androgen receptor (AR) belongs to a nuclear receptor superfamily and functions as a ligand-dependent transcription factor. AR-regulated transcriptional program is associated with various diseases, especially prostate cancer. Suppression of AR is an effective therapy for AR-dependent prostate cancer. However, in most of the cases it relapses as castration-resistant prostate cancer (CRPC), acquiring resistance to hormone therapy. We investigated the effect of androgen treatment and AR knockdown on gene expression by RNA-seq using CRPC model cells. Overall design: We treated CRPC model cells with dihydrotestosterone (DHT) or vehicle (ethanol) and siRNA targeting AR or drug (ribavirin: R).