Human spleen organoid single-cell trancsriptomics, enriched for γδ T cells

Vaccine effectiveness against malaria is dramatically reduced in
malaria-exposed compared to malaria-naïve populations, potentially due to
altered immune responses in secondary lymphoid organs following repeated
infection. Newly developed human tonsil and spleen organoids, which
replicate key features of B and T cell immunity, provide an exciting
opportunity to overcome challenges of other models and to improve our
understanding of innate-adaptive interactions in lymphoid tissue. The
objectives of this study were to use these organoids to investigate the
impact of malaria parasites on 1) cells within lymphoid tissues and 2)
responses to a heterologous antigen. To better characterize the impact of
iRBC stimulation on human lymphoid reponses, we cocultured three human
spleen organoids with iRBC and uninfected red blood cells for 10 days. We
then enriched for Vd2+ cells using fluorescence-activated cell sorting,
and used the BD Rhapsody platform to generate multiomic single-cell data,
combining transcriptomic, T cell receptor sequence, and surface protein
expression data. Data contained in this repository represents the Seurat
object generated after alignment and cell calling, but before quality
control. We hope these data are useful for researchers investigating the
utility of organoids as a means of enabling tissue-specific immunity in
humans. Our experiments were done with post-autopsy human tissues,
following established protocols and institutional ethics review. We report
no conflicts of interest.