Rational Design and Synthesis for Versatile FRET Ratiometric Sensor for Hg2+ and Fe2+: A Flexible 8-Hydroxyquinoline Benzoate Linked Bodipy-Porphyrin Dyad

A flexible 8-hydroxyquinoline benzoate linked Bodipy-porphyrin dyad has been designed, synthesized, and characterized. Binding of this dyad with Hg2+/Fe2+ induced just the opposite (promoting/restraining) influence on energy transfer from the Bodipy donor to the porphyrin acceptor, resulting in a remarkably different ratio change of two signal emissions, endowing this dyad as the first Bodipy-porphyrin-based versatile fluorescence resonance energy transfer (FRET) ratiometric sensor for Hg2+ and Fe2+ ions, respectively.本研究设计、合成并表征了一种由8-羟基喹啉苯甲酸酯（8-hydroxyquinoline benzoate）柔性连接的Bodipy-卟啉二元复合物。该二元复合物与汞离子（Hg²⁺）、亚铁离子（Fe²⁺）结合后，对Bodipy供体向卟啉受体的能量转移产生了截然相反的调控作用（促进/抑制），进而使两种信号发射的比值产生显著差异，使得该二元复合物成为首个基于Bodipy-卟啉结构的多功能荧光共振能量转移（Fluorescence Resonance Energy Transfer，FRET）比率型传感器，可分别用于汞离子与亚铁离子的检测。