Intracellular levels of AR, PSA and GSK-3β in different prostate cancer cell lines.

Western blots were performed as described under Figure 1. Protein levels of AR, PSA, GSK-3β were quantified by densitometry and normalized to the corresponding actin levels (loading control). Results are expressed in relation to the corresponding LNCaP controls which were set at 100%. Each value represents the mean of 3 independent experiments ± standard deviation (p-values compared to corresponding LNCaP values:*p = 0.006;**p = 0.002;***p = 0.006;#p = 0.016;##p蛋白质免疫印迹（Western blots）实验按照图1记载的方法开展。雄激素受体（Androgen Receptor，AR）、前列腺特异性抗原（Prostate Specific Antigen，PSA）、糖原合成激酶3β（Glycogen Synthase Kinase 3β，GSK-3β）的蛋白水平通过光密度法进行定量，并以对应肌动蛋白（actin，上样内参）的水平完成标准化校正。实验结果以对应LNCaP对照组的相对值表示，将该对照组设为100%。每个数值均为3次独立实验的平均值±标准差（与对应LNCaP组比较的p值：*p = 0.006；**p = 0.002；***p = 0.006；#p = 0.016；##p