Sort-seq assays of single fluorescent protein biosensor libraries
收藏NIAID Data Ecosystem2026-03-12 收录
下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA732942
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资源简介：
Libraries of single fluorescent protein biosensor variants were constructed with variation in either the site of GFP insertion into a ligand-binding domain or the composition of amino acid linkers connecting domains. The pooled libraries were characterized by a massively parallel assay combining FACS and DNA sequencing. A maltose-binding protein domain-insertion library was screened in E. coli. A library of linker mutations to the pyruvate biosensor PyronicSF was screened in HEK 293T TetBxb1BFP "landing pad" cell line.
创建时间：
2021-05-26
