Cross-hybridization comparison between cRNA target and cDNA target

We used two RNA samples that differed only by a set of three hemoglobin transcripts to compare microarray cross-hybridization between two target preparation protocols. We found widespread cross-hybridization using standard cRNA target but substantially less cross-hybridization using cDNA target. Keywords: protocol comparison 2 protocols x 2 RNA samples x 3 replicates = 12 arrays.