Data from: Anopheles gambiae: metabolomic profiles in sugar-fed, blood-fed and Plasmodium falciparum-infected midgut

The mosquito midgut is a physiological organ essential for the nutrient acquisition as well as an interface that encounters various mosquito borne pathogens. Metabolomic characterization would reveal biochemical fingerprints that are generated by various cellular processes. The metabolite profiles of the mosquito midgut will provide an overview of the biochemical events in both physiological states and the dynamic responses to pathogen infections. In this study, the midgut metabolic profiles of Anopheles gambiae mosquitoes following feeding with sugar, human blood, mouse blood, and Plasmodium falciparum-infected human blood were examined. A mass spectrometry system coupled to liquid and gas chromatography produced a time series of metabolites in the midgut at discrete conditions (sugar feeding, 24hr and 48hr post normal blood and P. falciparum-infected blood feeding). Triplicates were included to ensure system validity. A total of 512 individual compounds were identified, 511 were assigned to 8 super-pathways and 75 sub-pathways. The dataset can be used for further inquiry into the metabolic dynamics of sugar and blood digestion and of malaria parasite infection.

蚊中肠是获取营养的关键生理器官，同时也是多种蚊媒病原体接触的界面。代谢组学（metabolomic）表征可揭示由各类细胞过程产生的生化指纹图谱。蚊中肠的代谢物谱能够全面呈现其生理状态下的生化事件，以及对病原体感染的动态应答过程。本研究针对取食蔗糖、人血、鼠血以及感染恶性疟原虫（Plasmodium falciparum）的人血的冈比亚按蚊（Anopheles gambiae）的中肠代谢谱展开检测分析。本研究采用液相色谱-气相色谱联用质谱系统，在不同实验条件下（蔗糖取食组、正常血液取食后24小时及48小时、恶性疟原虫感染血液取食后24小时及48小时）获取了中肠代谢物的时间序列数据。实验设置了三组平行重复以确保系统检测的可靠性。共计鉴定得到512种独立化合物，其中511种被归类至8个超通路与75个亚通路。本数据集可用于进一步探究蔗糖与血液消化的代谢动态，以及疟原虫感染过程中的代谢变化。