Data from: Resources for phylogenomic analyses of Australian terrestrial vertebrates

High throughput sequencing methods promise to improve our ability to infer the evolutionary histories of lineages and to delimit species. These are exciting prospects for the study of Australian vertebrates, a group comprised of many globally unique lineages with a long history of isolation. The evolutionary relationships within many of these lineages have been difficult to resolve with small numbers of loci and we now know that many lineages also exhibit substantial cryptic diversity. Here, we present a set of phylogenetically diverse transcriptome resources to enable exon-based sequence capture studies of Australian vertebrates, including transcriptome sequences for 4 species of birds, 4 frogs, 7 lizards and 7 mammals. We also use exon data from the marsupial transcriptomes we generated to examine an approach for choosing a moderate number (dozens or hundreds) of phylogenetically informative exons based on a single transcriptome sequence, and a relatively distant reference genome.

高通量测序（High-throughput Sequencing）技术有望提升我们推断物种支系演化历史、界定物种的能力。这为澳大利亚脊椎动物的研究带来了极具潜力的前景：该类群囊括诸多全球特有的演化支系，且经历了长期的隔离演化历史。此前，依托少量基因座（loci）难以解析诸多此类支系的演化关系，而如今我们已证实，不少支系还存在丰富的隐存多样性（cryptic diversity）。本研究公开了一组系统发育多样性丰富的转录组（Transcriptome）资源，旨在支持澳大利亚脊椎动物的外显子靶向序列捕获研究，其中包含4种鸟类、4种蛙类、7种蜥蜴与7种哺乳类的转录组序列。此外，我们借助本次构建的有袋类转录组外显子数据，评估了一种基于单份转录组序列与相对远缘参考基因组，筛选适量（数十至数百个）系统发育信息性外显子的研究方法。