Spatial post-clustering differential expression: protocol, scripts and per-replicate outputs
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Protocol (written and hashed before any stress result existed), dated amendments, simulation and analysis scripts, and per-replicate output files for an evaluation of the naive Wilcoxon test, count splitting and ClusterDE for differential expression between clusters of spots in spatial transcriptomics. The evaluation uses simulated one-domain and two-domain worlds, a dose-response over the strength of spatial structure in null genes, a mixture world, and real tissue (human dorsolateral prefrontal cortex, slice 151673) with artificial boundaries, thinning spike-in markers and spot-shuffled controls. See README.md.
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Zenodo创建时间:
2026-11-01



