AMPK alpha1 and alpha2 knockout and usage of alternative polyadenylation sites

Role of alternative polyadenylation in muscle development remains largely undetermined. Our WTTS-seq approach to capture 3'-end of RNAs clearly revealed alternaitve polyadenylation events responsible for reduced muscle mass in AMPK alpha1, but increased muscle mass in AMPK alpha2 knockout mice. Total RNA samples obtained from muscle tissue of knockout (KO) mice and wild-type (WT) mice were used to construct WTTS-seq libraries. Eight WTTS-seq libraries including two AMPK alpha1 KO mice, two AMPK alpha2 KO mice, two AMPK alpha1 WT mice and two AMPK alpha2 WT mice were involved in this submission.