Brightfield images: p176

This repository constains all the brightfield plate scans plate p176 for the project: <strong>A quantitative analysis of the interplay of environment, neighborhood, and cell state in 3D spheroids</strong> Vito RT Zanotelli<br> Matthias Leutenegger<br> Xiao‐Kang Lun<br> Fanny Georgi<br> Natalie de Souza<br> Bernd Bodenmiller <em>Mol Syst Biol. (2020) 16: e9798</em><br> https://doi.org/10.15252/msb.20209798 <em>Please cite this article if you re-use any of the data or code.</em> Each plate acquisition follows the naming scheme: '{date}-{operator}-Spheroid-{plate}[rev]-{dps}-ac{acnr}_Plate_{acquisitionid} eg. 20180203-Vito-Spheroid-p173-2dps-ac1_Plate_13146 20180204-Vito-Spheroid-p173rev-3dps-ac1_Plate_1315 With: date: acquisition date operator: microscope operator plate: plate number rev: optional, if there indicates that the plate has been rotated 180 degree. E.g. well A01 will be well H12. dps: days post seeding ac: manual acquisition number (e.g. if acquisition needed to be repeated) acquisitionid: machine provided unique acquisition id Each folder contains: HTD files which contains acquisition metadata in text form. TimePoint_1: a folder containing all the acquisitions in .tiff form with the suffix _{wellname}_w{channel} In this acquisition 3 channels were used, which correspond to 3 brightfield images at 3 different z positions. An ImageXpress Micro XL Widefield High Content Imaging microscope (Molecular Devices, 4x objective, NA 0.20) was used for the acquisition.本仓库包含项目**《三维球体中环境、微环境与细胞状态间相互作用的定量分析》（A quantitative analysis of the interplay of environment, neighborhood, and cell state in 3D spheroids）**的全部明场平板扫描图像p176。作者依次为Vito RT Zanotelli、Matthias Leutenegger、Xiao‐Kang Lun、Fanny Georgi、Natalie de Souza、Bernd Bodenmiller，该研究发表于《分子系统生物学》（*Mol Syst Biol.*, 2020, 16: e9798），DOI链接：https://doi.org/10.15252/msb.20209798。若您复用本数据集或代码，请务必引用该文献。 所有平板采集文件遵循如下命名格式：`{采集日期}-{操作者}-Spheroid-{平板编号}[rev]-{接种后天数}-ac{采集序号}_Plate_{采集ID}`，示例如下： `20180203-Vito-Spheroid-p173-2dps-ac1_Plate_13146` `20180204-Vito-Spheroid-p173rev-3dps-ac1_Plate_1315` 各字段含义说明如下： - 采集日期：图像采集的具体日期 - 操作者：显微镜操作人员姓名 - 平板编号：实验所用平板的编号 - rev（可选字段）：若文件名携带该后缀，表示平板已旋转180°，此时原A01孔位将对应H12孔位 - 接种后天数（days post seeding, dps）：细胞接种后的培养天数 - ac：手动采集序号，用于需重复采集的场景 - 采集ID：仪器自动生成的唯一采集标识符 每个采集文件夹包含以下内容： 1. HTD文件：以文本格式存储采集相关的元数据 2. TimePoint_1文件夹：存储所有采集得到的TIFF格式图像，文件命名后缀为`_{孔位名称}_w{通道号}`。本次采集共启用3个通道，分别对应3个不同z轴位置的明场图像。 本实验采用ImageXpress Micro XL宽场高内涵成像显微镜（Molecular Devices公司，4×物镜，数值孔径NA 0.20）完成图像采集。