Additional file 1 of Apolipoprotein C1 stimulates the malignant process of renal cell carcinoma via the Wnt3a signaling

Additional file 1: Figure S1. Relative Protein expression of APOC1 in 12 representative paired cases of RCC and adjacent non-tumoral tissues. (T: RCC tissues, N: adjacent non-tumoral tissues), *P &lt; 0.05, error bars indicate mean ± SD, t test. Figure S2. Relative Protein expression of Wnt3a, β-catenin, CCND1 and TCF7 in CAKI-1 and 769P cell lines with APOC1 knockdown, *P &lt; 0.05, error bars indicate mean ± SD, t test. Figure S3. Relative Protein expression of Wnt3a, β-catenin, CCND1 and TCF7 in CAKI-1 and 769P cell lines overexpressing APOC1, *P &lt; 0.05, error bars indicate mean ± SD, t test. Figure S4. Wnt3a was partially responsible for APOC1-induced aggravation of RCC. (a) Protein level of Wnt3a and β-catenin in 769P cell intervened by Wnt3a and APOC1; (b) Viability in 769P cell intervened by Wnt3a and APOC1; (c, d) Migration and invasion in 769P cell intervened by Wnt3a and APOC1. *P &lt; 0.05, **P &lt; 0.01, error bars indicate mean ± SD, t test.附加文件1：图S1。载脂蛋白C1（APOC1）在12例代表性配对肾细胞癌（RCC）组织及其癌旁非肿瘤组织中的相对蛋白表达水平。（T：肾细胞癌组织，N：癌旁非肿瘤组织），*P < 0.05，误差棒表示均值±标准差（SD），采用t检验。 图S2。在APOC1敲低的CAKI-1与769P细胞系中，Wnt3a、β-连环蛋白（β-catenin）、细胞周期蛋白D1（CCND1）及转录因子7（TCF7）的相对蛋白表达水平，*P < 0.05，误差棒表示均值±标准差，采用t检验。 图S3。在APOC1过表达的CAKI-1与769P细胞系中，Wnt3a、β-连环蛋白、CCND1及TCF7的相对蛋白表达水平，*P < 0.05，误差棒表示均值±标准差，采用t检验。 图S4。Wnt3a部分介导APOC1诱导的肾细胞癌恶性进展。(a) 经Wnt3a与APOC1干预的769P细胞中，Wnt3a及β-连环蛋白的蛋白水平；(b) 经Wnt3a与APOC1干预的769P细胞的细胞活力；(c、d) 经Wnt3a与APOC1干预的769P细胞的迁移与侵袭能力。*P < 0.05，**P < 0.01，误差棒表示均值±标准差，采用t检验。