Mus musculus breed:C57BL/6J Raw sequence reads. Mus musculus breed:C57BL/6J

In principle, dominant mutations in the rhodopsin gene—the major cause of autosomal dominant retinitis pigmentosa—could be edited in patients by homologous recombination (HR) or nonhomologous end joining (NHEJ) of targeted genomic breaks. To explore these potential therapeutic strategies, we constructed zinc finger nucleases (ZFNs), a class of engineered nucleases that can bind at a specific DNA sequence and introduce a double strand break (DSB) at that site. This in vivo sequence data is investigating the frequency of cuts and repair events at the site of induced DSB