Impact of hypothermic preservation of mouse kidney resident immune cells. Mus musculus

With the growing interest in studying primary tissue samples by single cell transcriptome analysis, there is an emerging demand for a preservation strategy that enables sample transportation and storage. In this study, we describe a simple and general strategy that preserves primary tissues at hypothermic temperature. Using FACS and single-cell RNAseq, we demonstrated the effectiveness of this strategy in maintaining cell viability, cell population heterogeneity, and cell transcriptome integrity for primary tissues that underwent up to 3 days of preservation. Overall design: Examine the impact of hypothermic preservation on mouse kidney resident immune cells over up to 4 days at single-cell resolution