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Bacteria in microplastic biofilms have a distinct permissiveness to acquisition of antibiotic resistance genes in aquatic ecosystems

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NIAID Data Ecosystem2026-05-25 收录
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In this study, we analyzed the plasmid transfer permissiveness of freshwater bacteria forming biofilms on MP vs. those free-living (FL). We performed mating experiments using as a donor-plasmid system an exogenous E. coli carrying a gfp-tagged broad-host-range plasmid encoding for Trimethoprim resistance. We determined the transfer frequency and taxonomy of the transconjugants using epifluorescence microscopy, flow cytometry, and 16S rRNA gene sequencing.

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2018-05-01
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