Single-Cell Analysis of Age-Related Changes in Leukocytes of Diabetic Mouse Hindpaws

We used single cell RNA sequencing to identify transcriptomic changes in immune cells of the distal limb in diabetic Leprdb/db mice at two different ages that have the potential to contribute to the complications of the distal limb associated with diabetes Single cell RNA sequencing to identify transcriptomic changes in immune cells of the distal limb in diabetic Leprdb/db mice at two different ages. Cells were isolated from mice hindpaws and performed fluorescence-activated cell sorting (FACS) to separate the immune cells and then performed scRNAseq