Spain BAG for the 3D structure determination and dynamics of biological and non-biological samples using SSX and TR-SSX methods.

This Spanish BAG proposal, composed of eight groups from several research institutes in structural biology and MOF, is a collaborative effort with the main goal of forming a strong core of scientists dedicated to serial crystallography research in Spain. Our goal is to carry out SSX and TR-SSX experiments at ID29. We will study the reaction mechanisms and dynamics in a variety of therapeutically relevant enzymes including PBP2a from methicillin-resistant Staphyloccocus aureus, and the human enzymes NQO1, IMPDH and GMPR; as well as bacterial and plant-type ferredoxin NADP+ reductases such as FPR, FNR and Fld, and the FFTR enzyme from cyanobacteria. We also propose to perform pump-probe TR experiments on relevant materials such as MOFs. The quality of all crystalline samples has been tested at ESRF beamlines, including ID29 and ID23, or at other synchrotron beamlines. ID29 offers a variety of sample delivery devices to be used in our experiments depending on sample requirements.