Analysis of residual virus infectivity after pressure-induced inactivationa.

a The residual infectivity of the pressurized virus samples (282.7 MPa for 12 h at 25°C) was assayed for three sequential serial passages in embryonated hens eggs and in MDCK cell monolayers. For each blind passage, the samples revealed the absence of infectivity by a hemagglutination assay or a TCID50.b No residual infectivity was detected in MDCK cells.Analysis of residual virus infectivity after pressure-induced inactivationa.a. 针对经25℃、282.7 MPa压力处理12小时的病毒样品，通过在鸡胚（embryonated hens eggs）与MDCK细胞单层（Madin-Darby Canine Kidney cell monolayers）中进行三次连续系列传代，对其残余感染性进行测定。每一轮盲传均通过血凝试验（hemagglutination assay）或TCID₅₀（50% Tissue Culture Infectious Dose）检测，未检出样品的感染性。b. 在MDCK细胞中未检测到残余感染性。压力诱导灭活后的残余病毒感染性分析。