Escherichia coli AD30 Genome sequencing and assembly. Escherichia coli AD30

During our study of the Escherichia coli population structure in domestic animals, several E. coli strains were isolated from feces of cows, chickens and pigs. In an attempt to reveal any genetic relationship linked to hosts, whole genome sequencing of the isolated strains was performed using next-generation sequencing technology. The draft genome sequences were determined using a combination of shotgun sequencings from a paired-end (insert size = 8 Kb) in a Roche Genome Sequencer FLX system and from a paired-end in an Illumina Genome Analyzer IIx. The raw sequencing reads were assembled by CLC Genomics wb4, Newbler assembler 2.3 and CodonCode Aligner. The resultant contigs were further assembled using our own in silico assembly method. The ORFs were predicted using Glimmer 3 and annotated by searching against the in-house database constructed based on NCBI RefSeq DB.