遇见数据集

RNA-seq Raw Data from Wild-Type UM6 and Mutant Strain UM120 at 30°C and 37°C

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Zenodo2025-08-22 更新2026-05-26 收录
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This dataset contains the complete transcriptomic analysis outputs for the project X204SC23111202-Z01-F002. The data were generated using RNA-seq from Rhizopus microsporus, including three biological replicates per condition. The four experimental conditions were: Wild-type UM6 grown at 30 °C (UM630) Wild-type UM6 grown at 37 °C (UM637) choC mutant UM120 grown at 30 °C (UM12030) choC mutant UM120 grown at 37 °C (UM12037) The repository includes: RawData folderContains the raw sequencing data for all biological replicates. Each condition (UM630, UM637, UM12030, UM12037) is represented by three replicates (R1–R3). Each replicate folder includes two paired-end FASTQ files, corresponding to forward and reverse reads, as well as an MD5.txt checksum file. Compressed HTML reportIncludes quality control summaries, count tables, and interactive plots, viewable in any web browser. These data correspond to the analyses reported in the manuscript "Phosphatidylcholine Biosynthesis via choC is Crucial for Cellular Integrity and Virulence in Rhizopus microsporus". The final processed results used in the analyses presented in the manuscript are provided as Supplementary Material in the associated article

本数据集包含项目X204SC23111202-Z01-F002的完整转录组分析输出结果。数据源自米根霉(Rhizopus microsporus)的RNA测序(RNA-seq),每个实验条件设置3次生物学重复。本次实验共设置4组处理条件: 1. 30℃下培养的野生型UM6菌株(UM630) 2. 37℃下培养的野生型UM6菌株(UM637) 3. 30℃下培养的choC突变株UM120菌株(UM12030) 4. 37℃下培养的choC突变株UM120菌株(UM12037) 本数据集仓库包含以下内容: 1. RawData文件夹:存储所有生物学重复的原始测序数据。4组实验条件(UM630、UM637、UM12030、UM12037)各对应3次生物学重复(R1–R3)。 2. 每个生物学重复的文件夹内均包含两份双端FASTQ格式文件(分别对应正向测序读段与反向测序读段),以及一份MD5.txt校验文件。 3. 压缩HTML报告:包含质控汇总结果、基因计数表格与交互式可视化图表,可通过任意网页浏览器打开查看。 本数据集对应已发表论文《通过choC途径合成磷脂酰胆碱对米根霉的细胞完整性与致病性至关重要》中的相关分析内容。论文中所呈现分析所用的最终处理后结果,已作为补充材料附于相关正式发表文章中。

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创建时间:
2025-08-22
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