Sterol profiles of tobacco HMGS-OE seedlings and leaves ( µg/mg dry weight).

Two independent lines for each OE genotype were analysed. For OE-wtBjHMGS1, transformants “401” and “402” were tested. For OE-S359A, transformants “603” and “606” were tested. a indicates significant difference between HMGS-OE and the vector (pSa13)-transformed control; b indicates significant difference between OE-wtBjHMGS1 and OE-S359A. Bold font indicates significant higher sterol content than vector (pSa13)-transformed control and/or the OE-wtBjHMGS1 (Pt-test). Values are mean ±SD, n = 5.本研究针对每一种过表达（OE）基因型设置2个独立转化株系开展分析。针对OE-wtBjHMGS1基因型，测试了转化株系"401"与"402"；针对OE-S359A基因型，测试了转化株系"603"与"606"。字母a标注代表HMGS-OE与载体（pSa13）转化的对照组之间存在显著差异；字母b标注代表OE-wtBjHMGS1与OE-S359A之间存在显著差异。加粗字体代表经Pt检验后，其甾醇含量显著高于载体（pSa13）转化的对照组和/或OE-wtBjHMGS1株系。数据以平均值±标准差（Standard Deviation, SD）表示，每组n=5。