Expression data from cultured mouse spermatogonia cryopreserved on the International Space Station (ISS) or on Earth

Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes by supplementing GDNF and FGF2 (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and their gene expression profiles were analysed.

精原干细胞（Spermatogonial stem cells, SSCs）通过自我更新分裂以维持精子发生过程。本研究将SSCs应用于太空飞行环境下的长期冷冻保存实验。研究人员通过添加胶质细胞源性神经营养因子（GDNF）与成纤维细胞生长因子2（FGF2），从DBA/2小鼠睾丸中建立了生殖系干细胞（germline stem cell, GS）体外培养体系（Kanatsu-Shinohara等，2003，《生殖生物学》（Biol. Reprod.），第69卷，216-616页）。将所获得的GS细胞在国际空间站（International Space Station, ISS）中冷冻保存6个月。待返回地球后复苏冻存的GS细胞，并对其基因表达谱进行了分析。