Expression of quantitative trait loci analysis.

Background/ObjectivesObesity is a chronic metabolic disorder, and its prevalence in Bangladesh is increasing at an alarming rate. Previous reports have suggested a significant association between Vitamin D receptor (VDR) gene polymorphisms and obesity, but with inconsistent results. The purpose of our study was to investigate the association between two single-nucleotide polymorphisms (SNPs) (Apal, rs7975232, and Taql, rs731236) of the VDR gene and the risk of obesity in the Bangladeshi population. Moreover, we looked at serum VDR levels and serum 25-hydroxy vitamin D levels in people with obesity (n = 124) and healthy controls (n = 126).MethodsGenotyping was performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). General linear model and multivariate logistic regression analysis were used to calculate the adjusted odds ratio (OR) along with 95% confidence intervals (CI) and P-values.ResultsSerum VDR level was downregulated in people with obesity compared to healthy controls (P A) polymorphism, the CA Heterozygous genotype carried a 1.93-fold higher risk of developing obesity (OR=1.93, 95% CI = 1.10–3.41, P = 0.023). On the contrary, for TaqI, rs731236 (T > C), no significant association was found for both heterozygous and mutant homozygous genotypes.ConclusionWe report the downregulation of serum VDR levels and serum 25-hydroxy vitamin D levels in people with obesity. Moreover, a polymorphism of Apal (rs7975232 C > A) in the VDR gene increases the risk of developing obesity in the Bangladeshi population.研究背景与目的：肥胖是一种慢性代谢性疾病，其在孟加拉国的患病率正以惊人的速度增长。既往研究提示维生素D受体（Vitamin D receptor, VDR）基因多态性与肥胖存在显著关联，但研究结果并不一致。本研究旨在探讨孟加拉国人群中VDR基因的两种单核苷酸多态性（single-nucleotide polymorphisms, SNPs）——Apal（rs7975232）与TaqI（rs731236）与肥胖发病风险的关联。此外，本研究还检测了124例肥胖患者与126例健康对照者的血清VDR水平及血清25-羟维生素D水平。 研究方法：采用聚合酶链式反应-限制性片段长度多态性（Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, PCR-RFLP）方法进行基因分型。采用广义线性模型与多因素logistic回归分析计算校正后的比值比（odds ratio, OR）、95%置信区间（confidence interval, CI）及P值。 研究结果：与健康对照者相比，肥胖患者的血清VDR水平显著下调（P < 0.001）。针对Apal（rs7975232 C>A）多态性，CA杂合基因型携带者的肥胖发病风险升高1.93倍（OR=1.93，95%CI=1.10~3.41，P=0.023）。与之相反，针对TaqI（rs731236 T>C）位点，无论是杂合基因型还是突变纯合基因型，均未发现与肥胖发病风险存在显著关联。 研究结论：本研究证实肥胖患者的血清VDR水平及血清25-羟维生素D水平均出现下调。此外，孟加拉国人群中VDR基因的Apal（rs7975232 C>A）多态性会升高肥胖的发病风险。