Additional tables for "Quantitative catalogue of mammalian mitotic chromosome-associated RNAs"

We present comprehensive and quantitative characterizations of full-length mitotic chromosome-associated RNAs (mCARs) in three human cell lines (A549, ARPE-19, HT-1080), one monkey cell line (CV-1), and one mouse cell line (STO). This characterization was achieved through high-depth RNA sequencing. The enriched mCARs were identified using genomic DNA-based quantification of RNA-seq data, while the identification of unannotated RNAs relied on de novo assembly. Here the detail information of tables are shown below.Table S1_List of annotated mCARs in ARPE-19 ( Table S1-S1), A549 (Table S1-S2), HT-1080 (Table S1-S3), and CV-1 (Table S1-S4), STO (Table S1-S5).Table S2_List of Novel mCARs in ARPE-19 (Table S2-S1), A549 (Table S2-S2), HT-1080 (Table S2-S3), and CV-1 (Table S2-S4), STO (Table S2-S5).Table S3_The conversion factors needed to convert the measured TPM fold-change ratio to the proper enrichment factor for each cell-type. The final conversion factor is obtained by multiplying the slope of the qPCR fold-change vs TPM fold-change relationship (Supplementary Fig. 3) by 1/(Fraction retained).Table S4_The primers of the target RNAs for the qPCR in human, mouse, and monkey cells.

本研究针对三种人类细胞系（A549、ARPE-19、HT-1080）、一种猴细胞系（CV-1）及一种小鼠细胞系（STO）中的全长有丝分裂染色体相关RNA（mitotic chromosome-associated RNAs，mCARs）开展了全面且定量的表征。该表征基于高深度RNA测序（RNA-sequencing，RNA-seq）完成：富集的mCARs通过基于基因组DNA的RNA-seq数据定量方法鉴定，而未注释RNA的鉴定则依托从头组装（de novo assembly）技术实现。

下表为各补充表格的详细信息：
表S1：各细胞系中已注释mCARs的列表，分别对应ARPE-19（表S1-S1）、A549（表S1-S2）、HT-1080（表S1-S3）、CV-1（表S1-S4）及STO（表S1-S5）。
表S2：各细胞系中新发现的mCARs的列表，分别对应ARPE-19（表S2-S1）、A549（表S2-S2）、HT-1080（表S2-S3）、CV-1（表S2-S4）及STO（表S2-S5）。
表S3：将各细胞系中测得的TPM（Transcripts Per Million）倍数变化比值转换为标准化富集因子所需的转换系数。最终转换系数通过将qPCR（quantitative polymerase chain reaction）倍数变化与TPM倍数变化的相关性斜率（补充图3）乘以1/(保留分数)得到。
表S4：人类、小鼠及猴细胞中qPCR实验所用的靶RNA引物序列。