Supplemental Figure 1

Effects of heat stress and dietary organic acid and pure botanical supplementation on productive performance, milk composition and feed efficiency of pregnant multiparous lactating Holstein cows. Body weight (A), live weight variation (B), total water intake (C), 3.5% FCM (D), milk lactose percent (E), milk lactose yield (F), total solids percent (G), somatic cell count (SCC; H), milk efficiency (I), ECME (J), and 3.5% ECME (K). Cows were randomly assigned to one of four treatments at enrollment: unsupplemented thermoneutral conditions (TN-Con, n = 12), heat-stress with no organic acids and pure botanicals (OA/PB) supplementation (HS-Con, n = 12), thermoneutral conditions pair-fed to HS-Con (TN-PF, n = 12), HS supplemented with OA/PB (75 mg/kg of body weight [BW]; AviPlus R; VetAgro S.p.A., Italy; HS-OAPB, n = 10). Control cows (not supplemented with OA/PB) received a matching dose of the lipid matrix. BL: baseline values for acclimation period.

热应激与日粮添加有机酸及纯植物提取物（Organic Acid and Pure Botanicals, OA/PB）对妊娠经产泌乳荷斯坦奶牛（Holstein）生产性能、乳成分及饲料利用效率的影响。本试验测定指标包括：体重（A）、体重变化量（B）、总饮水量（C）、3.5%乳脂校正奶（3.5% Fat-Corrected Milk, 3.5% FCM, D）、乳乳糖百分含量（E）、乳乳糖产量（F）、总乳固体百分含量（G）、体细胞计数（Somatic Cell Count, SCC; H）、产奶效率（I）、能量校正乳当量（Energy-Corrected Milk Equivalent, ECME, J）及3.5%能量校正乳当量（3.5% ECME, K）。试验牛只入组时被随机分配至4个处理组：无添加外源添加剂的热中性环境对照组（TN-Con, n=12）、未添加OA/PB的热应激对照组（HS-Con, n=12）、与HS-Con组采食水平匹配的热中性配对饲喂组（TN-PF, n=12）、添加OA/PB的热应激组（HS-OAPB, n=10；OA/PB添加剂量为75 mg/kg体重（Body Weight, BW），产品为意大利VetAgro S.p.A.公司的AviPlus®）。未添加OA/PB的对照组牛只均饲喂等剂量的脂质载体。BL：适应期基线值。