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Primers used in PCR reactions for the detection of three target sequences in porcine organ, white blood cell and digesta samples.

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Primers_used_in_PCR_reactions_for_the_detection_of_three_target_sequences_in_porcine_organ_white_blood_cell_and_digesta_samples_/380362
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1PCR conditions for Sh-F & R included 1 cycle at 94°C for 3 min, 30 cycles of 94°C for 30 s, down to 55°C for 30 s and back up to 72°C for 45 s and 1 cycle of 72°C for 5 min; cry1Ab-F & R; 1 cycle at 94°C for 3 min, 32 cycles of 95°C for 30 s, down to 64°C for 30 s and back up to 72°C for 30 s and 1 cycle of 72°C for 5 min; SW-F & R; 1 cycle at 94°C for 1 min, 30 cycles of 94°C for 30 s, down to 60°C for 30 s and back up to 72°C for 40 s and 1 cycle of 72°C for 3 min.
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2015-12-02
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