Primers used in PCR reactions for the detection of three target sequences in porcine organ, white blood cell and digesta samples.

1PCR conditions for Sh-F & R included 1 cycle at 94°C for 3 min, 30 cycles of 94°C for 30 s, down to 55°C for 30 s and back up to 72°C for 45 s and 1 cycle of 72°C for 5 min; cry1Ab-F & R; 1 cycle at 94°C for 3 min, 32 cycles of 95°C for 30 s, down to 64°C for 30 s and back up to 72°C for 30 s and 1 cycle of 72°C for 5 min; SW-F & R; 1 cycle at 94°C for 1 min, 30 cycles of 94°C for 30 s, down to 60°C for 30 s and back up to 72°C for 40 s and 1 cycle of 72°C for 3 min.针对Sh-F与Sh-R的聚合酶链式反应（Polymerase Chain Reaction，PCR）条件如下：先于94℃预变性1个循环，时长3分钟；随后进行30个循环：94℃变性30秒，降温至55℃退火30秒，再回升至72℃延伸45秒；最后于72℃终延伸1个循环，时长5分钟。针对cry1Ab-F与cry1Ab-R的PCR条件为：94℃预变性1个循环，时长3分钟；随后进行32个循环：95℃变性30秒，降温至64℃退火30秒，再回升至72℃延伸30秒；最后于72℃终延伸1个循环，时长5分钟。针对SW-F与SW-R的PCR条件为：94℃预变性1个循环，时长1分钟；随后进行30个循环：94℃变性30秒，降温至60℃退火30秒，再回升至72℃延伸40秒；最后于72℃终延伸1个循环，时长3分钟。