Leishmania_braziliensis_gp63_functional_domain_sequence 5.fas

DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 5. The stretch of gp 63 previously described as encoding the functional domain of the molecule was amplified by PCR, cloned into pcr 2.1 vector and bidirectionally sequenced. Sequences from a panel of 41 parasite isolates were aligned and 45 alleles of the fragment could be identified based on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms (indel and SNPs) that could be detected in at least two clones of the gp63 domain in a single isolate, and in at least two parasite isolates in the collection were considered alleles. Corte de Pedra is a region highly endemic for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several forms of ATL occur and close to 1,500 new cases are diagnosed in the region each year.

来自巴西东北部科特德佩德拉（Corte de Pedra）的巴西利什曼原虫（Leishmania braziliensis）自然分离株中，编码利什曼蛋白酶（leishmanolysin）功能结构域的gp63片段的DNA序列：等位基因5。此前被报道可编码该蛋白功能结构域的gp63片段区域，经聚合酶链式反应（Polymerase Chain Reaction, PCR）扩增后，被克隆至pCR2.1载体并完成双向测序。对41株寄生虫分离株的序列进行多序列比对后，基于克隆结构域间存在的插入缺失变异（insertion-deletion, indel）与单核苷酸多态性（Single Nucleotide Polymorphisms, SNPs），可鉴定出该片段的45个等位基因。若某一多态性（含插入缺失变异与单核苷酸多态性）的单倍型，可在单株分离株的至少2个gp63结构域克隆中被检测到，且在该收集株系的至少2株寄生虫分离株中存在，则将其界定为等位基因。科特德佩德拉是由巴西利什曼原虫引发的美洲皮肤利什曼病（American tegumentary leishmaniasis, ATL）高流行区域。该地区存在多种临床类型的美洲皮肤利什曼病，每年新确诊病例近1500例。