Oral mucosa transplantation may improve tear film osmolarity in dogs with keratoconjunctivitis sicca - a preliminary study

ABSTRACT The objective of this study was to evaluate the use of cyclosporine 1% alone or associated with oral mucosa transplantation (OMT) in dogs with dry keratoconjunctivitis (KCS). Schirmer Tear Test (STT-1) and Tear Film Osmolarity (TFO) were measured in both eyes of 30 adult dogs (before and 45 days after treatment. The animals were divided into three groups (10 dogs for group): control (normal dogs), group I (GI, treated with 1% cyclosporine alone), and group II (GII, treated with 1% cyclosporine and OMT). All STT-1 and TFO values were subjected to the Shapiro-Wilk normality test, and all were normally distributed. STT-1 and TFO values before and after treatment were subjected to the T-Student Test. The STT‐1 and TFO values of the right eye were subjected to Repeated Measures ANOVA followed by a Tukey Test for comparison between groups I and II. Means with a value of p≤0.05 were considered significant. There was a decreased osmolarity in both groups after treatment. Mean osmolarity in GII (322.60±16.56 mOsm/L) was significantly lower than GI (336.40±5.66 mOsm/L). The OMT associated with cyclosporine 1% improved the osmolarity of the tear film in dogs with KCS with a seeming synergism between the clinical and surgical treatments.

摘要 本研究旨在评估单独使用1%环孢素（cyclosporine 1%）或联合口腔黏膜移植术（oral mucosa transplantation, OMT）治疗犬干性角膜结膜炎（dry keratoconjunctivitis, KCS）的效果。对30只成年犬的双眼分别进行泪液分泌试验（Schirmer Tear Test, STT-1）与泪液渗透压（Tear Film Osmolarity, TFO）检测，检测时点分别为治疗前及治疗后45天。将受试动物分为3组（每组10只犬）：对照组（健康正常犬）、Ⅰ组（GI，单独使用1%环孢素治疗）及Ⅱ组（GII，联合使用1%环孢素与OMT治疗）。对所有STT-1及TFO检测值进行夏皮罗-威尔克正态性检验，结果显示所有数据均符合正态分布。将治疗前后的STT-1与TFO值进行配对t检验。针对右眼的STT-1及TFO值，采用重复测量方差分析，随后通过图基多重比较检验对Ⅰ组与Ⅱ组进行组间比较。以p≤0.05作为差异具有统计学意义的判定标准。治疗后两组的泪液渗透压均有所降低。Ⅱ组的平均泪液渗透压（322.60±16.56 mOsm/L）显著低于Ⅰ组（336.40±5.66 mOsm/L）。联合使用1%环孢素与OMT可改善犬干性角膜结膜炎患犬的泪液渗透压，提示临床治疗与外科手术疗法间存在潜在协同效应。