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Effects on gene expression by Aff3-deficiency

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193735
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To analyze the effect of Aff3-deficeincy on gene expression in B cells, we employed cDNA microarray analysis. The data indicated that the deficiency altered the expressions of very limited number of genes. CD43-negative B cells were purified from Aff3+/- or Aff3-/- mouse spleen, and cultured in the presence of 10 ug/mL LPS and 10 ng/mL IL-4 for 3 days, and total RNA was extracted from these cells and used for the analysis.
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2023-01-17
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