Figure 4 of 'Structural basis for the specificity of PPM1H phosphatase for Rab GTPases'

Raw data for Figure 4 from manuscript 'Structural basis for the specificity of PPM1H phosphatase for Rab GTPases', Dieter Waschbüsch, Kerryn Berndsen, Pawel Lis, Axel Knebel, Yuko P. Y. Lam, Dario R. Alessi, Amir R. Khan

Figure 4A - sequences of the chimaeras.txt:

Aminoacid sequences of the chimaeric proteins used in the assays

Figure 4B.tif:
Coomassie stained gel of recombinant PPM phosphatases shown in Fig.4B. Last lane containing phosphatase PPM1A not shown in figure.

Figure 4CD Malachite.xlsx:

Numerical data for the charts shown in Fig.4C and Fig.4D.

Figure 4E 700.tif:
Top to bottom:

Total LRRK2 blot shown in Fig.4E

HA blot for PPM phosphatases (PPM constructs) shown In Fig.4E

GAPDH loading controls for blots in Fig.4E

HA blot for Rab8A shown in Fig.4E

Figure 4E 800.tif:

Phospho-Rab8A Thr72 blot shown in Fig.4E

Figure 4F 700.tif:
Top to bottom:

Total LRRK2 blot shown in Fig.4E

HA blot for PPM phosphatases (PPM constructs). HA blot was not used for Fig.4F. Membrane was reblotted, shown in tif file Figure 4F 700-2

GAPDH loading controls for blots in Fig.4F

HA blot for Rab10. HA blot was not used for Fig.4F. Membrane was reblotted, shown in tif file Figure 4F 700-2

Figure 4F 700-2.tif:
Top to bottom:

HA blot for PPM phosphatases (PPM constructs) shown In Fig.4E

GAPDH loading controls for blots in Fig.4F

HA blot for Rab8A shown in Fig.4E

Figure 4F 800.tif:

Phospho-Rab10 Thr73 blot shown in Fig.4F