Six-week exercise training with dietary restriction altered gut microbiota in adolescents with obesity

Between July and August 2018, participants with obesity who were 9 to 16 years of age were recruited from a weight loss camp after a physical examination. In brief, participants were provided with a calorie-restricted but nutritionally complete diet based on their age.. After each defecation, the contents of the intestinal tract were placed in separate sterile fecal collection tubes (5 mL), and liquid nitrogen was immediately poured into the tubes for preservation.