Speciation and adulteration analysis of milk by liquid AP-MALDI MS profiling

AP-MALDI MS profiling coupled with multivariate analysis for speciation and the detection of food adulteration using a simple one-pot sample preparation of milk. Milk samples from cow, goat, sheep and camel were classified with 100% accuracy. With respect to milk adulteration, it was possible to detect goat milk adulterated with 5% cow milk with 92.45% sensitivity and 94.53% specificity. Goat milk adulterated with 10% cow milk was detected with 99.15% sensitivity and 99.10% specificity.

采用简便一锅法牛奶样品前处理，结合大气压基质辅助激光解吸电离质谱（AP-MALDI MS）表征与多变量分析，用于牛乳来源物种鉴别及食品掺假检测。针对牛乳、山羊乳、绵羊乳及骆驼乳的样本分类准确率可达100%。在牛奶掺假检测场景中，对于掺入5%牛乳的山羊乳掺假样本，该方法的检测灵敏度为92.45%，特异性为94.53%；对于掺入10%牛乳的山羊乳掺假样本，其检测灵敏度可达99.15%，特异性可达99.10%。