Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 21/10/2009 to 25/10/2009 (Hobart - Dumont d'Urville)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 21/10/2009 to 25/10/2009 on a trip from Hobart to Dumont D'Urville.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于L'Astrolabe号研究船（平台代码：FHZI），采集时段为2009年10月21日至2009年10月25日，航次为从霍巴特前往迪蒙·迪尔维尔站。

光系统II（photosystem II, PSII）最大光化学效率，亦称PSII最大量子产额（Fv/Fm），已成为浮游植物研究中应用最为广泛的荧光参数之一。该参数表征潜在光化学效率，即光合装置捕获的光能用于光化学反应的概率。已有研究表明，Fv/Fm对水体理化性质变化具有瞬时响应，可作为浮游植物整体健康状态与生存能力的诊断指标。结合PSII吸收截面面积与叶绿素浓度数据，该参数可用于水体初级生产力的测定（Cheah等，2011，《深海研究》）。

本研究采用船舶洁净海水管路连续采集3米深度的海水样品。在暗适应状态（需经15分钟暗适应）下，以1分钟为间隔连续测定快速重复率荧光（Fast Repetition Rate, FRR）产额；测定所用闪光序列包含两组闪光：100个亚饱和微闪（闪光时长1.1 μs，闪光间隔2.8 μs）与20个弛豫微闪（闪光时长1.1 μs，闪光间隔51.6 μs）。