Supplementary tables used in this study.
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Table S1. ImageStream raw data used in Fig. 1. Table S2. DESeq2 results of RLRb high versus RLRb low immunostaining shown in Fig. 1F. Table S3. GSEA results shown in Fig. 1G. Table S4. DESeq2 results of mCherry positive versus mCherry negative sorted cells. Used in Fig. 3 H-j. Table S5. GSEA results shown in Fig. 3J. Table S6. UMI fraction per metacell. Used for Fig. 5B. Table S7. Differential expression analysis of poly(I:C) versus NaCl treated embryos using MAST on scRNAseq data. Table S8. Marker genes reported by Cole et al. (48) Table S9. Cell cluster markers identified in this study. Used in fig. S6. Table S10. MAST differential expression for cluster 1 and GSEA results. Table S11. WGCNA analysis for identifying gene modules (fig. S7) Table S12. ORA results for GS17 (brown) gene module. Table S13. ORA results for GS16 (lightcyan) gene module. Table S14. ORA results for GS14 (red) gene module. Table S15. ORA results for GS15 (purple) gene module. Table S16. Marker genes identified in sub-clusters 0-3. Used in Fig. 6. Table S17. ORA results for sub-cluster 0. Table S18. ORA results for sub-cluster 1. Table S19. ORA results for sub-cluster 2. Table S20. ORA results for sub-cluster 3. Table S21. Orthofinder output used for orthogroups identification in Fig. 7. Table S22. Shared single orthogroups and their gene expression used for correlation analysis in Fig. 7D Table S23. Shared genes with adjusted p value < 0.05 used for correlation analysis in Fig. 7E. Table S24. Genes that were shared between cluster in the non-injected control and the immune cluster in adult Nematostella identified by Cole et al. (48). Table S25. Dictionary for converting NVE gene models, NV2, and the gene models used in this study and their associated annotations.
表S1 用于图1的ImageStream原始数据 表S2 对应图1F的RLRb高免疫染色组与RLRb低免疫染色组的DESeq2差异表达分析结果 表S3 对应图1G的基因集富集分析（Gene Set Enrichment Analysis, GSEA）结果 表S4 mCherry阳性分选细胞与mCherry阴性分选细胞的DESeq2差异表达分析结果，用于图3H-J 表S5 对应图3J的GSEA结果 表S6 每个元细胞（metacell）的唯一分子标识符（Unique Molecular Identifier, UMI）占比，用于图5B 表S7 基于单细胞RNA测序（single-cell RNA sequencing, scRNAseq）数据，使用基于单细胞转录组的模型分析（Model-based Analysis of Single-cell Transcriptomics, MAST）对聚肌胞苷酸（poly(I:C)）处理组与氯化钠（NaCl）处理组的胚胎进行的差异表达分析 表S8 Cole等人（48）报道的标记基因 表S9 本研究鉴定的细胞簇标记基因，用于补充图S6 表S10 1号细胞簇的MAST差异表达分析结果及GSEA富集分析结果 表S11 用于鉴定基因模块的加权基因共表达网络分析（Weighted Gene Co-expression Network Analysis, WGCNA）结果，对应补充图S7 表S12 GS17（棕色）基因模块的过度表征富集分析（Overrepresentation Analysis, ORA）结果 表S13 GS16（浅青色）基因模块的ORA富集分析结果 表S14 GS14（红色）基因模块的ORA富集分析结果 表S15 GS15（紫色）基因模块的ORA富集分析结果 表S16 亚簇0-3中鉴定得到的标记基因，用于图6 表S17 0号亚簇的ORA富集分析结果 表S18 1号亚簇的ORA富集分析结果 表S19 2号亚簇的ORA富集分析结果 表S20 3号亚簇的ORA富集分析结果 表S21 用于图7中同源基因组（orthogroups）鉴定的Orthofinder分析结果 表S22 用于图7D相关性分析的共有单同源基因组及其基因表达数据 表S23 用于图7E相关性分析、校正后P值<0.05的共有基因集 表S24 Cole等人（48）鉴定得到的非注射对照组细胞簇与成年海葵免疫细胞簇间的共有基因 表S25 用于转换NVE基因模型、NV2及本研究使用的基因模型及其对应注释的词典文件
