Images of selected cells of the TbGOS02 recombinant Trypanosoma brucei brucei cell line

A novel experimental setup for measuring fluorescence in live trypanosome (FILT) cells was established as described in Borg (2015) and Borg et al. (2021), a study which will be linked to this dataset upon publication. The FILT set up was used to capture 60 fluorescent and 60 brightfield images of a field of vision which encompassed 88 cells of the recombinant <i>Trypanosoma brucei brucei</i> cell line, TbGOS02. All these images are provided in the file within this dataset labelled, "TbGOS02 All Frames 88 cells 140619.pdf".<br> Each of the 88 cells in the image was enumerated as detailed in Borg et al. (2021). For cell number 08, 60 fluorescent and 60 brightfield cropped images featuring only this cell are provided in the file within this dataset labelled, " TbGOS02 All Frames Cell 08 140619.pdf ". Equivalent images for cells numbered 17, 63 and 76 are provided in the remaining three PDF files of this dateset, as summarised below. <b>TbGOS02 All Frames 88 cells 140619.pdf</b>All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of the 88 cells in view field. <b>TbGOS02 All Frames Cell 08 140619.pdf</b>All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 08.<b> </b><b>TbGOS02 All Frames Cell 17 140619.pdf</b>All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 17 <b>TbGOS02 All Frames Cell 63 140619.pdf</b>All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 63 <b>TbGOS02 All Frames Cell 76 140619.pdf</b>All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 76<br> References<i>Borg Y (2015). Design, Mathematical Modelling, Construction and Testing of Synthetic Gene Network Oscillators to Establish Roseobacter Clade Bacteria and the Protozoan Trypanosoma brucei as Synthetic Biology Chassis. Doctoral thesis, University College London. https://discovery.ucl.ac.uk/id/eprint/1472672</i><i> </i><i>Borg Y, Alsford S, Pavlika V, Zaikin A, Nesbeth D (2021). Synthetic biology tools for engineering Goodwin oscillation in Trypanosoma brucei brucei. In preparation.</i>

本研究按照Borg（2015）及Borg等人（2021）中的方法，搭建了一套用于活体锥虫荧光测量（Fluorescence in Live Trypanosome, FILT）的新型实验装置，该研究成果正式发表后将与本数据集关联。

本FILT装置用于采集视野内的图像，该视野包含重组布氏布氏锥虫（Trypanosoma brucei brucei）细胞系TbGOS02的88个细胞，共获得60张荧光图像与60张明场图像。上述所有图像均存储于本数据集内名为"TbGOS02 All Frames 88 cells 140619.pdf"的文件中。

图像中的88个细胞均已按Borg等人（2021）中的细节完成编号。针对编号为08的细胞，本数据集提供了仅包含该细胞的60张荧光裁剪图像与60张明场裁剪图像，存储于名为"TbGOS02 All Frames Cell 08 140619.pdf"的文件中。

针对编号为17、63、76的细胞，本数据集剩余的三个PDF文件中提供了对应的等效图像，具体如下：

**TbGOS02 All Frames 88 cells 140619.pdf**：包含视野内88个细胞的全部120张由FILT装置采集的图像（60张荧光图像、60张明场图像）。
**TbGOS02 All Frames Cell 08 140619.pdf**：包含编号08细胞的全部120张由FILT装置采集的图像（60张荧光图像、60张明场图像）。
**TbGOS02 All Frames Cell 17 140619.pdf**：包含编号17细胞的全部120张由FILT装置采集的图像（60张荧光图像、60张明场图像）。
**TbGOS02 All Frames Cell 63 140619.pdf**：包含编号63细胞的全部120张由FILT装置采集的图像（60张荧光图像、60张明场图像）。
**TbGOS02 All Frames Cell 76 140619.pdf**：包含编号76细胞的全部120张由FILT装置采集的图像（60张荧光图像、60张明场图像）。

参考文献
[1] Borg Y. 合成基因网络振荡器的设计、数学建模、构建与测试：以玫瑰杆菌分支细菌与原生动物布氏锥虫作为合成生物学底盘. 伦敦大学学院博士学位论文, 2015. https://discovery.ucl.ac.uk/id/eprint/1472672
[2] Borg Y, Alsford S, Pavlika V, Zaikin A, Nesbeth D. 用于在布氏布氏锥虫中构建古德温振荡的合成生物学工具. 待发表, 2021.