Vertebral fusion anomalies in Nf1flox/flox and Nf1flox/−;PeriCre and Col2.3 mice.

Anterior-posterior and lateral radiographs of WT (n = 29), Nf1+/− (n = 18), Nf1flox/flox;PeriCre (n = 46), Nf1flox/−;PeriCre (n = 41), Nf1flox/flox;Col2.3Cre (n = 15), and Nf1flox/−;Col2.3Cre (n = 20) mice were acquired to screen for the presence of vertebral fusion anomalies. The percentage of mice exhibiting vertebral fusion defects as well as the frequency distribution of the affected segments are summarized in the table and pie chart below.*Fusion present at L4–5 in all 4 mice**Fusion present at multiple spinal levels: T13-L1 = 2 mice, L1–2 = 1 mouse, L3–4 = 3 mice, L4–5 = 6 mice, L5–6 = 2 mice, L6-S1 = 1 mouse#Fusion present at L4–5 in 1 mouse##Fusion present at multiple spinal levels: T10–12 = 1 mouse, T12–13 = 2 mice, L2–3 = 2 mice, L3–4 = 3 mice, L4–5 = 3 mice, L5–6 = 1 mouseVertebral fusion anomalies in Nf1flox/flox and Nf1flox/−;PeriCre and Col2.3 mice.