Genome-Scale Patterns in The Loss of Heterozygosity Incidence in Saccharomyces cerevisiae

We applied extensive assays--repeated over several genetic backgrounds and environments--to reveal some general features of the loss of heterozygosity (LOH) in yeast. We found that LOH: was most frequent in homozygotes, was more densely distributed on shorter chromosomal arms, depended on the presence of some repetitive elements but not the intensity of transcription, occured also in absence of growth although at a low rate. Our findings uncover new features of LOH and help to interpret those already known. Overall design: Examination of transciption levels of BY4743 Saccharomyces cerevisiae strain in six different conditions; 2 biological replicates for each sample