Quantification of AdVP transduction levels in hMSCs

Determining AdVP transduction efficiency in hMSCs. Conclusion: Transduction efficiencies, as determined by flow cytometry, varied from a minimum of 92.4±2.2% to a maximum of 99.23±0.8%. Notes: The hMSCs were exposed to the indicated amounts of AdVP.mCherry. At 3 days post-transduction, the frequencies of transduced cells and the protein expression levels per transduced cell, were assessed by mCherry-directed flow cytometry. Untransduced mock cells served to establish fluorescence background thresholds. At least 10,000 viable single cells were acquired per sample