Assessment of the effect of RNase A-treated Enterococcus feacalis strain EC-12(EC-12) or untreated EC-12 on transcriptome profiles in human peripheral blood mononuclear cells

Whole blood was collected from healthy adult and peripheral blood mononuclear cells (PBMC) were isolated. PBMCs were cultured in medium or stimulated with RNase treated EC-12 or untreated EC-12 for 6hours. Then transcriptome profiles in PBMC were obtained. Comparison: control PBMC (medium) vs PBMC situmulated with untreated EC-12, and control PBMC vs PBMC situmulated with RNase-treated EC-12, PBMC stimulated with untreated EC-12 vs PBMC stimualted with RNase treated EC-12. Biological replicates; 3 donors; 2 control(medium), 3 untreated EC-12, 3 RNase-treated EC-12.