SMARCD2 neutrophil chemotaxis

Defective chemotaxis of SMARCD2-deficient patient cells assessed with TaxiScan. Travel control and patient cells stimulated with C5a (100 nM; Video 1 = travel control and 2 = patient) or fMLF (100 nM; Video 3 = travel control and 4 = patient). TAXIScan analysis was performed with an EZ-TAXI scan. Data accompany the manuscript "Specific granule deficiency and myelodysplasia caused by a homozygous splice site mutation in SMARCD2" by Ina Schim van der Loeff, Evelien G.G. Sprenkeler, Anton T.J. Tool, Mario Abinun, Angela Grainger, Karin R. Engelhardt, Michel van Houdt, Hans Janssen, Taco W. Kuijpers, and Sophie Hambleton, to be published in the Journal of Allergy and Clinical Immunology.

本数据集通过TaxiScan（TAXIScan）平台评估SMARCD2缺陷患者细胞的趋化功能缺陷。以100 nM的C5a刺激迁移对照细胞与患者细胞（视频1对应迁移对照样本，视频2对应患者样本）；或以100 nM的fMLF刺激上述细胞（视频3对应迁移对照样本，视频4对应患者样本）。实验采用EZ-TAXI scan系统完成TaxiScan分析。本数据集配套的研究论文为《SMARCD2纯合剪接位点突变所致特异性颗粒缺乏症与骨髓增生异常》，作者包括Ina Schim van der Loeff、Evelien G.G. Sprenkeler、Anton T.J. Tool、Mario Abinun、Angela Grainger、Karin R. Engelhardt、Michel van Houdt、Hans Janssen、Taco W. Kuijpers及Sophie Hambleton，即将发表于《Journal of Allergy and Clinical Immunology》。