Defining the interplay of carbon dioxide and the carbonic anhydrase CanB in regulating Mycobacterium tuberculosis PhoPR signaling and virulence

Regulation of M. tuberculosis gene expression by carbon dioxide at acidic and neutral pH. M. tuberculosis CDC1551 cultures were seeded at a starting OD600 of 0.2 in 8 mL of 7H9 buffered media and grown at 37°C in standing T-25 culture flasks. Two biological replicates of the following culture conditions were examined: i) 0.5% CO2 at pH 5.7, ii) 5% CO2 at pH 5.7, , iii) 0.5% CO2 at pH 7.0, iv) 5% CO2 at pH 7.0. Cultures were incubated for six days, after which total bacterial RNA was extracted.