synthetic construct Raw sequence reads. synthetic construct

As part of this study, several experiments were conducted comparing the effects of PCR amplification method (TAS or DePCR) and annealing temperature (45C or 55C) on fidelity of polymerase template copying and amplification. Each experiment was a PCR amplification of synthetic DNA templates, ranging from a single template to a combination of up to 10 different templates. In some experiments, synthetic DNA templates were added to the PCR reaction mixture at equimolar concentration, while in others, each template was added at a different concentration. In addition to varying input templates, a set of 64 primers were used in different combinations to amplify the synthetic templates. In some reactions, only a single primer was used, while in others, various combinations of primers were used to amplifying synthetic DNA templates.