Percent composition of butachlor and metabolites as determined by GC-MS analysis.

The GC-MS measurements were carried out with the metabolites extracted from the reaction mix containing 50mM potassium phosphate buffer pH 7.4, 0.4 mM butachlor and 10mM 2-mercaptoethanol, (1) neither NADPH nor AKR, (2) NADPH (0.12mM), (3) AKR (5 μg), or (4) both NADPH (0.12mM) and AKR (5 μg).采用气相色谱-质谱联用仪（GC-MS）对从含50mM磷酸钾缓冲液（pH 7.4）、0.4mM丁草胺及10mM 2-巯基乙醇的反应体系中提取的代谢物进行检测，实验设置四组条件：(1) 既未添加烟酰胺腺嘌呤二核苷酸磷酸（NADPH）也未添加醛酮还原酶（AKR）；(2) 仅添加NADPH（0.12mM）；(3) 仅添加AKR（5μg）；(4) 同时添加NADPH（0.12mM）与AKR（5μg）。